Plants monitor and respond to their environment constantly, which is essential for their viability and fitness. The ultimate goal of our research is to understand the molecular mechanisms by which plants perceive environmental signals and integrate signals to regulate their growth and development. We are using Arabidopsis thaliana as a model plant to investigate two distinct but interacting responses, one to temperature variations and one to pathogen invasions.
The first focus of our current research is to investigate how immune receptor R genes are regulated at the transcriptional level. R genes mediate the perception of pathogens and activate defense responses in plants. Constitutive expression or activation of R genes due to the loss of negative regulation of R genes triggers autoimmune responses. We are using these autoimmune mutants to genetically and molecularly dissect the signaling mechanisms that control R gene expression.
The second focus of our research is to investigate the interplay between temperature and plant immunity. Plant defense responses to pathogens are influenced by abiotic factors including temperature. An elevated temperature often inhibits disease resistance, however, molecular mechanisms of this modulation is not well understood. We are using Arabidopsis mutants that have altered temperature sensitivity in disease resistance to reveal components subject to temperature regulation and to understand the interaction between biotic and abiotic responses.
I teach plant development, plant molecular biology laboratory, undergraduate research, and graduate modules.
- Hua, J. (2013). Modulation of plant immunity by light, circadian rhythm, and temperature. Current Opinion in Plant Biology. 16:406-413.
- Bao, Z., Yang, H., & Hua, J. (2013). Perturbation of cell cycle regulation triggers plant immune response via activation of disease resistance genes.. Proceedings of the National Academy of Sciences of the United States of America.
- Zhu, Y., Du, B., Qian, J., Zou, B., & Hua, J. (2013). Disease resistance gene-induced growth inhibition is enhanced by rcd1 independent of defense activation in Arabidopsis.. Plant Physiology. 161:2005-13.
- Mang, H., Qian, W., Zhu, Y., Qian, J., Kang, H., Klessig, D. F., & Hua, J. (2012). ABA deficiency antagonizes high temperature inhibition of disease resistance through enhancing nuclear accumulation of R proteins SNC1 and RPS4.. Plant Cell. 24:1271-1284.
- Zhu, Y., Mang, H., Sun, Q., Hipps, A., & Hua, J. (2012). Gene discovery using mutagen-induced polymorphisms and deep sequencing: application to plant disease resistance.. Genetics. 192:139-146.
- Mingyue, G., Zhenying, S., Ying, Z., Zhilong, B., Guoying, W., & Hua, J. (2012). The F-box protein CPR1/CPR30 negatively regulates R protein SNC1 accumulation. Plant Journal. 69:411-420.
- Li, Y., Gou, M., Sun, Q., & Hua, J. (2010). Requirement of calcium binding, myristoylation, and protein-protein interaction for the copine BON1 function in Arabidopsis. Journal of Biological Chemistry. 285:29884-29891.
- Zhu, Y., Qian, W., & Hua, J. (2010). Temperature modulates plant defense responses through NB-LRR proteins. PLoS Pathogens. 6:e1000844.
- Wang, Y., & Hua, J. (2009). A moderate decrease in temperature induces COR15a expression through the CBF signaling cascade and enhances freezing tolerance. Plant Journal. 60:340.
- Yang , S., Hua, J., & , (2004). A haplotype-specific Resistance gene regulated by BON1 mediates temperature-dependent growth control. Plant Cell. 16:1060-1071.
- Hua, J., Grisaffi , P., Cheng, S. H., & Fink , G. R. (2001). Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes. Genes Dev. 15:2263-2272.
- Hua, J., & Meyerowitz, E. M. (1998). Ethylene responses are negatively regulated by a receptor gene family. Cell. 94:261-271.
- Hua, J., Chang , C., Sun , Q., & Meyerowitz , E. M. (1995). Ethylene insensitivity conferred by Arabidopsis ERS gene. Science. 269:1712-1714.